Pfu DNA Polymerase

NP041011420 - 500 U
NP041011410 - 1000 U

Pfu DNA Polymerase is a thermostable enzyme with a molecular weight of 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5'_3' direction, resulting in blunt-ended PCR products without 3'-dA overhangs.
Pfu DNA Polymerase exhibits 3'_5' exonuclease (proofreading) activity that enables the polymerase to correct the mis-incorporation of nucleotide, and lacks 5'_3' exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter than 3 kbp. The enzyme exhibits 3´>5´ proofreading activity, resulting in over 10-fold higher PCR fidelity than possible with Taq DNA Polymerases.

	NP041011420	NP041011410
Components	500U	1000 U
Pfu DNA poly. 5 u μl	500U	2 x 500U
MgCl2 Solution 25 mM	1 mL	2 x 1 mL
5X Pfu Buffer MgCl2 free	1 mL	2 x 1 mL

Pfu DNA Polymerase

Pfu DNA Polymerase

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