Hot-start Taq DNA Polymerase

NP041010120 - 500 U
NP041010110 - 1000 U

This DNA polymerase is a mixture of Taq DNA polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40 °C, but releases the enzyme during normal PCR cycling conditions. The aptamer-based hot start mechanism does not require a separate high temperature incubation step to activate the enzyme. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The activated enzyme maintains the same functionality as Taq DNA polymerase: it catalyzes 5' → 3' synthesis of DNA, has no detectable 3' → 5' proofreading exonuclease activity.

	NP041010120	NP041010110
Components	500 U	1000 U
HS Taq DNA poly. 2.5 U/μl	500 U	2 x 500 U
MgCl2 Solution 25 mM	1 mL	2 x mL
10X Buffer MgCl2 free	1 mL	2 x 1 mL

Hot-start Taq DNA Polymerase

Hot-start Taq DNA Polymerase

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