Pfu DNA Polymerase

NP040114500 - 500 U
NP040114102 - 1000 U

Pfu DNA Polymerase is a thermostable enzyme with a molecular weight of 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5'_3' direction, resulting in blunt-ended PCR products without 3'-dA overhangs.

Pfu DNA Polymerase exhibits 3'_5' exonuclease (proofreading) activity that enables the polymerase to correct the mis-incorporation of nucleotides, and lacks 5'_3' exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter than 3 kbp. The enzyme exhibits 3´>5´ proofreading activity, resulting in over 10-fold higher PCR fidelity than possible with Taq DNA Polymerases.

Components

	NP040114500	NP040114102
Components	500U	1000 U
Pfu DNA poly. 5 u μl	500U	2 x 500U
MgCl2 Solution 25 mM	1 mL	2 x 1 mL
5X Pfu Buffer MgCl2 free	1 mL	2 x 1 mL

Pfu DNA Polymerase

Pfu DNA Polymerase

Would you like to benefit from our experience right away?