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PURIFICATION

PURIFICATION SERVICE

Subject

Details

Required Information

  • Full name of the protein, NCBI or Uniprot ID
  • Amino acid sequence of the protein (optional)
  • Functional role of the protein (if known)
  • Source of the protein (e.g., E. coli, cell culture, tissue)
  • Estimated purity and concentration of the protein
  • Other requirements: labeling, special purification material
  • Name of the antibody target
  • Type of antibody (e.g., polyclonal, monoclonal)
  • Source of the antibody (e.g., rabbit, goat, mouse)
  • Estimated purity and concentration of the antibody
  • Other requirements: labeling, conjugation, etc.

Protocol

  • Analysis of protein source and selection of appropriate purification method (e.g., chromatography, precipitation).
  • Purification of the protein and determination of purity and concentration.
  • Purified protein provided in the desired format (e.g., buffer solution, lyophilized).
  • Analysis of antibody source and selection of appropriate purification method (e.g., protein A/G chromatography, affinity chromatography).
  • Purification of the antibody and determination of purity and concentration.
  • Purified antibody provided in the desired format (e.g., buffer solution, lyophilized).

Quality Control

  • SDS-PAGE electrophoresis
  • Measurement of antibody or protein concentration
  • ELISA assay
  • Western blot test.

Time

  • Protein purification: 2-4 weeks (depending on the protein)
  • Antibody purification: 3-5 weeks (depending on the type and source of the antibody)

Provided Products

  • Purified protein or antibody (10-50 mg) (May vary depending on antibody or protein)
  • Purification report

Price

  • Prices may vary depending on the required quantity or complexity of the protein or antibody. Please contact us for pricing information. Measurement of antibody or protein concentration.

Others

  • We offer purification service for different types of proteins and antibodies.
  • We also offer protein and antibody labeling services.
  • For more information, please contact us.

Please note: The above information provides a general overview. Please contact us for specific requirements. Prices may vary depending on the complexity of the project and the required quantity of protein. Your privacy and intellectual property rights are important to us. All your information will be kept confidential.

 

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